Introduction Flyers

Magnetic bead based MagSi-DNA cleanFIX offers one single product for efficient PCR clean-up and Dye terminator removal from DNA Sanger sequencing reactions, enabling researchers to address various genomic clean-up steps with the same product. MagSi-DNA cleanFIX uses flexible protocols and is easy to automate for high-throughput processing.

Scientists can use Protein A or Protein G coated MagSi silica beads as an isolation tool for applications like total IgG purification, Immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), protein isolation etc. Direct cross-linking after binding of IgG molecules saves the user time and money.

MagSi-STA beads allows scientists to use streptavidin coated MagSi silica beads to immobilize biotinylated compounds for capturing targeted molecules. Strong read-out signals and lowered background noise enable scientists to create sensitive assays with improved dynamic range. Besides standard solutions MagnaMedics offers customized MagSi-STA products optimized according to client specifications.

MagSi-WAX is used for the fractionation of complex protein and/or peptide sample mixtures (e.g. serum, urine, cell lysates) using pH or salt concentration differences. MagSi-WAX proteins separation makes use of molecular difference in net charge, where beadbound charged groups reversibly adsorb oppositely charged molecules. The immobilized proteins or peptides are washed to remove contaminants and salts.Bound target material is easily collected with a magnet. MagSi-WAX and MagSi-WCX offer - with MagSi-proteomics - a second protein fractionation solution. MagSi-WAX can also be used for phosphorylated proteins & peptides enrichment.

MagSi-WCX are used for the fractionation of complex protein & peptide sample mixtures (e.g. serum, urine, cell lysates) using pH- or salt concentration differences. Protein & peptide separation with MagSi-WCX beads makes use of molecular difference in net charge, where bead-bound charged groups reversibly adsorb oppositely charged molecules. The immobilized proteins & peptides are washed to remove contaminants and salts. MagSi-WCX offer -beside MagSi-proteomics- a second, complementary protein & peptide fractionation solution.

Proteins & peptides bind MagSi-proteomics via hydrophobic adsorption interaction between the peptide groups to be isolated and the matrix-bound hydrophobic ligands (MagSi beads with C4-, C8- or C18-chains). The relative protein interaction ability depends upon the number of hydrophobic amino acids exposed to the medium and the beads. Proteins & peptides are separated according to their relative hydrophobicity using stepwise desorption in increasing concentrations of organic solvents. MagSi-proteomics offer, besides the MagSi-WCX and MagSi-WAX beads, a second protein isolation method.