Adrenocortical dysfunction

Cortisol is synthesized from cholesterol in the adrenal glands. It is the main glucocorticoid in humans and plays a critical role in glucose metabolism, maintenance of vascular tone, immune response regulation, and in the body's response to stress. Cortisone, a downstream metabolite of cortisol, provides an additional variable to assist in the diagnosis of various adrenal disorders, including abnormalities of 11-beta-hydroxy steroid dehydrogenase (11-beta HSD), the enzyme that converts cortisol to cortisone.

Determining the amount of biologically active, free cortisol in urine or saliva is a measure for the various types of adrenocortical dysfunction, particularly hypercortisolism (Cushing syndrome) and Addison's disease. LC-MS/MS measurements of 24-hour collected urine, or saliva collected with e.g. Salivette® have become established tests. LC-MS/MS methodology eliminates the analytical interferences which can affect immunoassay-based cortisol results.

Adrenal diseases          Sex hormone - Test

Endocrine disorders

Congenital Adrenal Hyperplasia (CAH) is a group of inherited disorders of the adrenal gland. CAH is associated with deficiencies in the enzymes required in the production of the steroid hormones cortisol and/or aldosterone. The enzyme defects result in reduced glucocorticoids and mineralocorticoids, and elevated 17-hydroxyprogesterone (17-OHP) and androgens. The resulting hormone imbalances can lead to life-threatening, salt-wasting crises in the newborn period and incorrect gender assignment of virilized females. Adult-onset CAH may result in hirsutism or infertility in females.

Determining the correct amounts of specific sets of steroid hormones can best be done by LC-MS/MS. Its methodology eliminates the analytical interferences which can affect immunoassay-based steroid results. Furthermore, only LC-MS/MS is able to offer high-quality multiplex results.

Now introducing: 2 new MagSiMUS-SteroidPREP kits

  • MagSiMUS-SteroidPREP (Cortisole/Cortisone for LC-MS)
    Urine and saliva sample preparation for LC-MS detection of cortisol and/or cortisone

  • MagSiMUS-SteroidPREP (Universal, for LC-MS)
    Serum sample preparation for LC-MS detection of more than 13 steroid hormones in panels

Request your sample here 

Art. No. Product  Volume
 MD03033 MagSiMUS-SteroidPREP (Cortisol/Cortisone for LC-MS)  500 preps
 MDKT00100500 MagSiMUS-SteroidPREP (Universal, for LC-MS)  500 preps
Kit components available separately / Accessory products
 MD71335 Organic Precipitation Reagent VI (OPR VI)  100 mL
 MD71530 Internal Standard Dilution Reagent B (ISDR B)  100 mL
 MD71730 Urine Stabilization Buffer (component of MD03033 only)  10 mL