The MagSi-gDNA blood kits allow fast and economic automated purification of genomic DNA from a wide range of sample volumes (20 μl up to 1 ml) of fresh or frozen whole blood samples using magnetic beads and the KingFisher® workstationa. The scalabillity of our MagSi gDNA blood kits makes it possible to use the products in any situation where gDNA isolation out of blood is needed from single experiments using the MM-Separators leading though to high throughput platforms of which the KingFisher® is a perfect and widely used example.

Advantages:

• 

  Ultra pure, ready to use genomic DNA for enhanced performance in sensitive applications
• Erythrocyte lysis is not necessary
• High binding capacity
• Reproducible yields from large blood volumes
• No cross contaminations
• Standardized processing ensures reproducible results
• Optimal preservation of gDNA
• Low amounts of (chemical) waste
• Ordering of single different kit components possible

In combination with the proven KingFisher® workstations the automated MagSi gDNA blood kits enables purification of DNA from 1 - 96 samples of whole human blood or other commonly used blood components (buffy coat, serum). After filling the standard containers (single tubes, 96 deepwell plates, all required isolation steps are robottically performed. In contrast to other blood kits available using blood volumes > 1 ml, lysis of erythrocytes is not required. Blood sample lysed with the MagSi gDNA blood kit is optimal as the lysis buffer contains Proteinase K.
In combination with the robotic workstation the lysis step can be carried out direct on the platform at ambient temperature or via the integrated thermomixer available for most robots at 56°C. Further processing is done by making use of the magnetic capabillities of the MagSi DNA beads.
The free DNA binds to magnetic particles, followed by washing steps and the final elution. The high quality DNA can be stored at –20 °C or is ready to use for subsequent downstream applications like:

• PCR, Real-time PCR
• RFLP analysis
• Restriction enzyme digestion
• Hybridisation
• SNP analysis
• Genotyping

DNA isolation from human whole blood
DNA was isolated from 200 μl human whole blood (Citrate stabilized) and 200 μl human whole blood
(EDTA stabilized) using the Magsi gDNA blood kit

lane 1-7 human whole blood (Citrate)
lane 9-15 human whole blood (EDTA)
lane 8 blank

"The MagSi-gDNA kit is only available for Resseller or OEM clients as bulk product."
In case you are interested in a Bulk delivery of this product please contact This e-mail address is being protected from spambots. You need JavaScript enabled to view it .