Protein Purification & Fractionation

Protein Purification

Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest. The purification process may separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps usually exploit differences in protein size, physico-chemical properties, binding affinity and biological activity. The pure result may be termed protein isolate.

MagSi-proteomics are magnetic silica particles with C4, C8 or C18 modified surface for sample preparation prior to e.g. mass spectrometry analysis. 

MagSi-WCX are magnetic silica particles with weak cation exchange surface (WCX). MagSi-WAX are magnetic silica particles with weak anion exchange surface (WAX). They are both ideal for the reduction of protein or peptide complexity. Applications include sample preparation and prefractionation prior to mass spectrometry or SDS-PAGE analysis, biomarker analysis and serum/ plasma profiling.