MagSi-DNA clean^FIX kit:
All your sequencing sample purifications on any scale in one single solution! 

MagnaMedics' one solution approach for DeoxyDye^TM terminator removal, enables researchers to make use of one kit for any type of sample numbers and sample volumes likely used in labs performing sequencing as part of their daily research routine. It addresses the practical need for a fast solution for any lab, any sample volume and any throughput.

Key features of the MagSi-DNA clean^FIX kit:
•    Single component kit, saving space in lab and fridge
•    Fixed protocols for 5-, 10 and 20 μl sample volumes
•    Suitable for single tube, 96- and 384-plate processing
•    Consistent quality of processed samples ensured
•    Short processing times in any case
•    Tailored protocols for individual robotic set-ups possible
•    Cost efficient solution in any circumstance

Comparing the commonly known techniques for DNA sequence sample cleaning, MagSi-DNA clean^FIX kit shows reduced processing times up to 6 fold!

Table 1: Comparison of clean up techniques used for  DeoxyDyeTM terminator removal

*based on the most extensive protocol published as in product manual MagSi-DNA clean^FIX kit

DNA cycle sequencing reactions
The MagSi-DNA clean^FIX kit is an efficient tool for removal of DyeDeoxy^TM terminators from DNA cycle sequencing reactions using ABI^TM terminator kits. Our MagSi-DNA clean^FIX kit solution enables researchers to process up to 384 samples in one plate. By using the patented MagSi nanoparticle technology, it offers an excellent opportunity to automate existing processes when dealing with large and growing amounts of samples in the lab.

Figure 2: Data proving the quality of our set-up. Details: 10 μl sequence sample, template used: pGEM as provided in ABI Big Dye kit V3.0, injection time ABI3730xl sequencing system: 13 seconds. Data kindly provided by Dr Jost Muth, Frauenhofer IME institute Aachen. Software: ABI Sequence scanner v1.0.

High quality DNA sequencing readout
The MagSi-DNA clean^FIX kit is designed to enable high quality DNA sequencing data read outs with the original ABI Sequence scanner v1.0 software. Readouts from approximately 20-30 bp to >700 bp can be achieved. The analysis in this case on sample F02_003 generates values of 964 bases, and these CRL-values are considered “long” in accordance with general accepted views.

Figure 3: Quality control data provided for F02_003 sample as shown in Figure 2.

Quality Control aspects of MagSi-DNA clean^FIX kit
Besides a visual readout an important other parameters indicates the quality of the clean-up of crude sequence samples as well. As in the Legend (figure 3), Trace Score values > 20 are set as “high” in this standard set-up of the software. However many sequence core facilities appreciate and adopt much higher Trace Scores (> 40). With MagSi-DNA clean^FIX kit  on average the score for sample F02_003 is: 43, which is considered excellent under any circumstance of sequence clean up.

Download this overview as pdf document here

Download the protocol for the MagSi-DNA clean^FIX kit

Download the MagSi-DNA clean^FIX handling guidelines