Immunoprecipitation & IgG Purification

Immunoprecipitation & IgG Purification


Immunoprecipitation is the technique of precipitating a protein antigen out of solution using an antibody that specifically binds to that particular protein. This process can be used to isolate and concentrate a particular protein from a sample containing many thousands of different proteins. Immunoprecipitation requires that the antibody be coupled to a solid substrate at some point in the procedure.


Protein A and Protein G bind to Fc regions of immunoglobulins. After binding onto magnetic beads with a coating of Protein A or Protein G, immobilized immunoglobulins can be used for immunoprecipitation of various biomolecules, or can be eluted in a native or denatured state.