Protein A and protein G are recombinant proteins that bind with high affinity to the Fc portion of several classes and subclasses of immunoglobulin (IgG´s) from a variety of species. Specific antigens are bound in a second step reversible towards the bound IgG. A following washing step and elution by pH shift generates the purified target protein under native conditions. MagSi protein A and G beads can be regenerated and reused several times (up to 10 times), incl large volume scales.

The advantage of protein G and A beads, compared to streptavidin beads, is that the antibody does not need to be biotinylated and the binding is reversible. So they are ideal for protein isolation and proteomics applications. Furtheron the superior surface chemistry is blocking unspecific binding of residual proteins and further biomolecules. Therefore your target protein can be purified in a single purification step.

Click here to download the newsletter about MagSi-Protein A and G.